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SIZE PARAMETERS
INNER DIAMETER- The diameter of the maximum inscribed circle.
(maximum circle lying completely inside the particle)
AREA DIAMETER- (ED or EQUIVELANT DISC DIAMETER) diameter
of a circle having the same diameter as the particle.
WIDTH- Defined as the projection of the particle on the inertia elipse minor axis. These are Feret Diameters in the direction of the inertial elipse axes.
LENGTH- Defined as the projection of the particle on the inertia elipse major axis. These are Feret Diameters in the direction of the inertial elipse axes.
SHAPE PARAMETERS
LUMINANCE- The mean greyscale level of the particle. Value '0' corresponds to a black particle. As the value increases the particle becomes more clear.
BLUNTNESS INDEX- The expression of a,"maturity in the abrasion process", or how far we are from a perfectly rounded shape. Although it expresses the total amount of rough features, it does not give information as to the shape of the asperities. In other words, it does not give any indication as to the speed at which the rough features would wear off in an abrasion process. Bluntness is based on a very accurate measure of local curvature, and takes in to account the fact that very acute asperities wear off almost instantaneously, as compared to blunt ones.
ROUGHNESS INDEX- Defined as the amount of material to be removed from the shape before getting a smooth surface.Smoothness is gauged by a smooth reference set, which is the set of all inscribed discs having a diameter equal or greater
than 80% of the sieving diameter.
ELONGATION- Defined as (1-ASPECT RATIO) ,where the aspect ratio is the ratio between the width and length of the particle.
El = 1-(Width/Length)
CIRCULARITY- The ratio between the equivalent disc perimeter, and the particle perimeter. Values are in the range (0,1)
SOLIDITY- Object area divided by the area enclosed by the convex hull. The covex hull can be imagined as the border created by a rubber band wrapped around the object. The convexity lies in the range [0.0,1.0]. A convex shape has convexity 1.0, while a concave shape, has a lower value, closer to 0.
ABSOLUTE COUNTING OF PROTEINS SUSPENDED IN A THIN CELL USING
HIGH RESOLUTION IMAGING TECHNOLOGY
The Occhio FC200SHR is part of a family of highly sophisticated imaging systems, that are byproducts of a series of collaborative studies in light, imaging, and sample transport, in connection with the University De Liege in Belgium. The FC200SHR (FLOW CELL
200 SYRINGE PUMP HIGH RES), in particular, was developed to solve some of the current technological hurdles with protein aggregate counting.
LIMITATIONS OF EXISTING TECHNOLOGY
SUB MICRON DETECTION OF AGGREGATES
LIQUID SUB SAMPLING
AGGREGATE DIFFERENTIAL SPEEDS
HIGH RESOLUTION IMAGING TECHNOLOGY
Occhios' high resolution imaging technology, is accomplished in part, by using special backlighting techniques focused from behind the sample. An adjustable range, monochromatic, blue light source has been shown to provide optimal contrast conditions for the particles, and virtually eliminate diffraction. The light is collimated, and acts as a high definition caliper. In the case of liquids and suspensions, the light flashes simultaneously with a high speed, high definition camera, for extremely robust particle definitions.
(LEFT) A TELECENTRIC LENS IS USED WITH THE CAMERA.
COMPARISON SLIDES OF OPTICAL MICROSCOPY AND NEWLY DEVELOPED DIGITAL IMAGING TECHNIQUES
SUB MICRON DETECTION OF AGGREGATES-In order to improve the quality of the images, the Occhio FC200S HR uses a higher resolution camera, with an optical calibration of .38 microns per pixel, compared with an estimated 1.4 microns per pixel, on a Brightwell DPA 4200. The flow cell on an Occhio FC200S HR, is also half the width at 50 microns.
