ABSOLUTE COUNTING OF PROTEINS SUSPENDED IN A THIN CELL USING HIGH RESOLUTION IMAGING TECHNOLOGY
The Occhio FC200SHR is part of a family of highly sophisticated imaging systems, that are byproducts of a series of collaborative studies in light, imaging, and sample transport, in connection with the University De Liege in Belgium. 

The FC200SHR IPAC, in particular, was developed to solve some of the current technological hurdles with protein aggregate counting. 
LIMITATIONS OF EXISTING TECHNOLOGY


HIGH RESOLUTION IMAGING TECHNOLOGY

This high resolution imaging technology, is accomplished in part, by using special backlighting techniques focused from behind the sample. An adjustable range, monochromatic, blue light source has been shown to provide optimal contrast conditions for the particles, and virtually eliminate diffraction. The light is collimated, and acts as a high definition caliper. (Fig. 1.2) In the case of liquids and suspensions, the light flashes simultaneously with a high speed, high definition camera, for extremely robust particle definitions.
COMPARISON SLIDES OF OPTICAL MICROSCOPY AND NEWLY DEVELOPED DIGITAL IMAGING TECHNIQUES
SUB MICRON DETECTION OF AGGREGATES-In order to improve the quality of the images, the Occhio FC200S HR uses a higher resolution camera, with an optical calibration of .38 microns per pixel, compared with an estimated 1.4 microns per pixel, on a Brightwell DPA 4200. The flow cell on an Occhio FC200S HR, is also half the width at 50 microns.
OCCHIO FLOWCELL
COMPARISON USING LATEX SPHERES
SPECIAL SYRINGE PUMP FOR PRECISE SAMPLING.
SOLUTIONS TO TECHNOLOGY LIMITATIONS

SUB-MICRONIC DETECTION OF AGGREGATES


LIQUID SUB SAMPLING

AGGREGATE DIFFERENTIAL SPEED
OCCHIO FC200SHR PARTICLE CHARACTERISTIC ANALYZER FOR PROTEIN AGGREGATES
SUB VISIBLE PARTICLE AND AGGREGATE DETECTION TO 200 nm
OCCHIO FLOWCELL TYPE PARTICLE ANALYZER FOR LIQUIDS AND SUSPENSIONS WITH SYRINGE PUMP AND HIGH RESOLUTION CAMERA OPTION FC200S HR

DYNAMIC ANALYSIS
APPLICATION
COLLIMATED LIGHT SOURCE ACTS LIKE A CALIPER TO PROVIDE SUPERIOR PARTICLE DEFINITION IN REAL TIME
IMAGE MAGNIFICATION IS INDEPENDENT OF THE OBJECTS DISTANCE OR POSITION IN THE FIELD OF VIEW
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DRY POWDERS / GRANULARS       LESS DENSE (VACUUM TRANSPORT)         (20 µm-5 mm)


DRY POWDERS / GRANULARS       MORE DENSE (GRAVITY TRANSPORT)        (30 µm-30 mm)

LIQUID ANALYSIS CAPABILITIES OPTION                                                           (30 µm-1 mm)


DRY POWDERS AND GRANULARS                                                                      (0.4 µm-2 mm)

HIGH RESOLUTION OPTION (10 MILLION PIXELS)

LIQUID ANALYSIS CAPABILITIES OPTION                                                           (0.4 µm-1 mm)


LIQUIDS WITH  PARTICLES SUBJECT TO BREAKAGE                                        (0.8 µm-1mm)   

HIGH RESOLUTION OPTION (10 MILLION PIXELS)

PERILSTATIC PUMP  OPTION FOR PARTICLES > 500 µm


LIQUIDS WITH SMALLER PARTICLES                                                                 (0.2 µm-1mm) 

HIGH RESOLUTION OPTION (10 MILLION PIXELS)


SURFACES, FOAMS, PARTICLES                                                                       (10 µm - 50 mm)
SAMPLE CHARACTERISTICS CHECK ALL THAT APPLY
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SAMPLER CATALOGUE
OCCHIO FC200S IPAC FOR PROTEIN AGGREGATES